Antioxidant and Antibacterial Activities of Hydrolysate Chitooligosacharides in Crab Shell (Portunus Pelagicus) from Degradation of Chitosanase, α-Amylase, Lipase and Cellulase Enzymes

The crab shell (Portunus pelagicus) has many benefits, one of the benefits is used in manufacture chitooligosaccharides. was chosen because it contains 20- 30% chitin inside. Chitin can be processed into chitosan and then hydrolyzed chitooligosaccharides using enzymes. This study aims to determine type enzyme that effective process hydrolyzing with various enzymes are α-amylase, lipase, cellulase, chitosanase a combination cellulase chitosanase. observation data were analyzed ANOVA. results showed best obtained resulting from hydrolysis which had characteristics, namely highest degree deacetylation 94.95%, lowest molecular weight 3.54 KDa, antioxidant activity DPPH method 5.21 ( µmol TE/g), FRAP 2.27 (µmol diameter inhibition area (DDH) 7.30 mm Staphylococcus aureus bacteria 15.9 Salmonella typhosa bacteria. From these results, known this chitooligosaccharide more inhibiting growth. Gram-positive compared gram-negative